Post on 07-Apr-2016
Identificação de Identificação de levedurasleveduras
Taxonomia clássica
Extrato de malte
MacroscopiaSedimentoResíduoAnelIlhotaPelículaMicroscopiaForma e tamanhodas células
MicrocultivoAgar fubáTween 80Corn meal agar
MicroscopiaPseudomicélio,Micélio verdadeiro,BalistósporosClamidósporos
MicroscopiaProdução esporos sexuais:Ascos e ascósporos
Meio de acetatoGorodkowaYMÁgua de torneira
Outras provas:-Urease-Cápsula-Meio DBB
IDENTIFICAÇÃO GENÉRICA
AuxanogramaAssimilaçãoFontes C e N
ZimogramaFermentação Alcoólica de açúcares
Meios diferenciais-meio TTC-ágar molibdênio-ágar azul Tripan-meio de Nickerson-meio de CGB
Outras provas:-Crescimento 37ºC-Produção de compostos amilóides-tubo germinativo-resistência aCicloheximida-fenol oxidase
IDENTIFICAÇÃO ESPECÍFICA
The xylose-fermenting yeast, Pichia stipitis (A) during vegetative growth, (B) forming spores, and (C) under carbon limitation. (FPL photo-Tom Kuster)
2006 Highlights
o How Well Do Arsenic-Free Preservatives Inhibit Copper-Tolerant Fungi?
o
Caulking with Care o Combustion Properties of an Exotic Annual Grass o Creosote Movement from Treated Wood Immersed in Fresh Water o Durable Wood, Naturally--Termite Resistanceo Essential Oils Inhibit Mold Spore Germination o Fire Resistance of Strucutral Composite Lumber Products o Bioprocessing for Ethanol Production and a Valuable Adhesive Coproduct o
Fuel Treatment Evaluator o Fuel Treatment Marketo Genome Sequencing o Wood Research from World War I to Iraqo Reusing Remediated Wood in Value-Added Products o Inspecting Historic Structures: Using the Web to Train Inspection Professionals o Productive Use of Thinnings in Dimension Lumber and Papero Treatability of Underutilized Wood Species o Enhancing Wood-Plastic Composites by Crosslinking Polymerso Accessible and Affordable Playground and Path Surfacing Now Commerically Available o Dimensional Warping of Wood-Based Composites
The xylose-fermenting yeast, Pichia stipitis (A) during vegetative growth, (B) forming spores, and (C) under carbon limitation. (FPL photo-Tom Kuster)
2006 Highlights
o How Well Do Arsenic-Free Preservatives Inhibit Copper-Tolerant Fungi?
o
Caulking with Care o Combustion Properties of an Exotic Annual Grass o Creosote Movement from Treated Wood Immersed in Fresh Water o Durable Wood, Naturally--Termite Resistanceo Essential Oils Inhibit Mold Spore Germination o Fire Resistance of Strucutral Composite Lumber Products o Bioprocessing for Ethanol Production and a Valuable Adhesive Coproduct o
Fuel Treatment Evaluator o Fuel Treatment Marketo Genome Sequencing o Wood Research from World War I to Iraqo Reusing Remediated Wood in Value-Added Products o Inspecting Historic Structures: Using the Web to Train Inspection Professionals o Productive Use of Thinnings in Dimension Lumber and Papero Treatability of Underutilized Wood Species
o Enhancing Wood-Plastic Composites by Crosslinking Polymers
o Accessible and Affordable Playground and Path Surfacing Now Commerically Available
o Dimensional Warping of Wood-Based Composites
The xylose-fermenting yeast, Pichia stipitis (A) during vegetative growth, (B) forming spores, and (C) under carbon limitation. (FPL photo-Tom Kuster)
A. Haploid yeast cells buddingB. Haploid cells forming shmoos and zygotesC. Zygote budding off diploidD. Diploid buddingE. Diploid forming asci with ascospores; freed haploid spores
Teste do DBB. As linhagens CCA 181, 506 e 567 são DBB negativas, enquanto a CCA379 é DBB positiva (utilizada como controle positivo)
ESPOROSSEXUAIS
Produçãode pseudomicélio
Candida albicans - pseudomicélio
Carbono Nitrogênio
Lin
hage
m
Erit
ritol
S
acar
ose
Raf
inos
e G
alac
tose
X
ilose
M
elez
itose
A
rabi
nose
C
elob
iose
M
anito
l M
elib
iose
G
licos
e E
tano
l M
alto
se
Ac.
Lac
tico
Ac.
Citr
ico
Ram
nose
A
c. S
uccí
nico
In
osito
l L
isin
a
Etil
amin
a
Nitr
ato
005 - + + + + + + - - - + - + - - - - + - + - 006 - + + + + + + + + + + + + - + + + - + + - 007 - + - + + + + - - - + - + - - - - - - + - 008 - + + - - + + - - + + - + - - - - + - - - 012 - + + + + + + - - - + - + - - - + + + + - 022 - + - + - + - - + - + - - - - - - - - nd - 023 - + + + + + + - - + + - + - - - - + - - - 027 - + - + - + + - - - + - + - - - - - - nd - 035 - + - + + - + - + - + - + - - - - - - nd - 037 - + + - + + - - - + + - + - - - - + - - - 053 - + + + + + + + - - + - + - - + + + + + + 059 - + - - + + + + - - + - + - - - + + + + + 077 - + + + - + - + + - + + + - - - + + + + + 083 - + + + + + + - - - + - + - - - - + - - - 115 - + - + + + + - + - + + + - + - - + + + - 151 - + - - - - + - - - + - - - - - - - + - - 155 - + - + + + + + - - + + + - - + + + + + + 181 - + - + + + + + + - + + + - + - + + + + - 209 - + + + + + - - - + + - - - - - + - - - 373 - + - + + + + - + - + + + - - - + + - + - 385 - - - - - - + - - - + - + - - - - - - - - 397 - + - + - + + - - - + - - - - - + + - - - 447 - + - + + + + - + - + + + - + - + + + + - 467 - + - + + + + - + - + + + - + - + - + + + 471 - + - + + + + - + - + + + - + - + - + + - 506 - + - + + + + + + + + + + - + - + + + + - 540 - + - + nd + + - + - + + + - + - + + + + - 567 - + - + + + + - + - + + + - + - + + + + - 593 - + + + + + + - + - + + + - + - + + + + - 610 - + - - + + + + + - + + + - + - - + + + - 612 - + - + + + + + + - + + + - + - + + + + -
1 - (+) = positivo; (-) = negativo e (nd) = não determinado;
Carbono Nitrogênio
Lin
hage
m
Erit
ritol
Sac
aros
e R
afin
ose
Gal
acto
se X
ilose
Mel
ezito
se A
rabi
nose
Cel
obio
se M
anito
l M
elib
iose
Glic
ose
Eta
nol
Mal
tose
Ac.
Lac
tico
Ac.
Citr
ico
Ram
nose
Ac.
Suc
cíni
co In
osito
l L
isin
a
Etil
amin
a
Nitr
ato
005 - + + + + + + - - - + - + - - - - + - + - 006 - + + + + + + + + + + + + - + + + - + + - 007 - + - + + + + - - - + - + - - - - - - + - 008 - + + - - + + - - + + - + - - - - + - - - 012 - + + + + + + - - - + - + - - - + + + + - 022 - + - + - + - - + - + - - - - - - - - nd - 023 - + + + + + + - - + + - + - - - - + - - - 027 - + - + - + + - - - + - + - - - - - - nd - 035 - + - + + - + - + - + - + - - - - - - nd - 037 - + + - + + - - - + + - + - - - - + - - - 053 - + + + + + + + - - + - + - - + + + + + + 059 - + - - + + + + - - + - + - - - + + + + + 077 - + + + - + - + + - + + + - - - + + + + + 083 - + + + + + + - - - + - + - - - - + - - - 115 - + - + + + + - + - + + + - + - - + + + - 151 - + - - - - + - - - + - - - - - - - + - - 155 - + - + + + + + - - + + + - - + + + + + + 181 - + - + + + + + + - + + + - + - + + + + - 209 - + + + + + - - - + + - - - - - + - - - 373 - + - + + + + - + - + + + - - - + + - + - 385 - - - - - - + - - - + - + - - - - - - - - 397 - + - + - + + - - - + - - - - - + + - - - 447 - + - + + + + - + - + + + - + - + + + + - 467 - + - + + + + - + - + + + - + - + - + + + 471 - + - + + + + - + - + + + - + - + - + + - 506 - + - + + + + + + + + + + - + - + + + + - 540 - + - + nd + + - + - + + + - + - + + + + - 567 - + - + + + + - + - + + + - + - + + + + - 593 - + + + + + + - + - + + + - + - + + + + - 610 - + - - + + + + + - + + + - + - - + + + - 612 - + - + + + + + + - + + + - + - + + + + -
1 - (+) = positivo; (-) = negativo e (nd) = não determinado;
Classificação das leveduras Reino Fungi
Divisão Eumycota Subdivisão Ascomycotina Subdivisão Basidiomycotina Subdivisão Deuteromycotina
Chaves de identificação Kreger-van Rij, N.J.W. The yeasts. A
taxonomic study. Elsevier, 1984.
Barnett, J.A., Payne, R.W., Yarrow, D. Yeasts: characteristics and identification. Cambridge Univ. Press, 1990.